Counterscreen assay
WebDetailed information for compound 1286650. Table of contents ; Basic information ; Compound Sub-Network ; Synonyms 7; Known and predicted targets WebThe assays used for HTS of hit compounds are called primary screen. Once hits have been confirmed in the primary screen, assays need to be designed to eliminate false positives caused by the effect of compounds on the assay technology or format. Counter-screen …
Counterscreen assay
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WebL1 (CTSL1) enzyme-based assay was developed and used as a counterscreen to identify compounds with nonspecific activity. Enzymology and phenotypic assays were used to determine mechanism of action and efficacy of selective and potent compounds identified from high-throughput screening. Two structurally related compounds, CID 6852389 and CID WebAug 23, 2024 · To mitigate this, we also developed a counterscreen assay that identifies potential interferers at the levels of both the luciferase enzyme reporter and the mechanism responsible for a glow-like response. Interestingly, we also found that the peptide substrate for chymotrypsin-like proteasome activity was not specific and was likely partially ...
WebDec 12, 2013 · Counterscreen for inhibitors of PfM17LAP: QFRET-based biochemical high throughput dose response assay for inhibitors of the Plasmodium falciparum M1AAP (PfM1AAP) (Counterscreen Assay AID … WebThe peptide-based counterscreen assay is conducted as follows: Figure 8.7. We developed an assay-based counterscreen that takes advantage of the biotinylated hexahistidine peptide including all nickel chelate AlphaScreen kits. This counterscreen format will reveal compound interference due to nickel chelation, excitation/emission …
WebAug 28, 2009 · The purpose of this assay is to determine the cytotoxicity of compounds identified as STAT3 inhibitor probe candidates. This assay also serves as a counterscreen to identify false positives and non-selective … WebJun 1, 2011 · Counterscreen Assay (elimination of false positives) The purpose of the counterscreen assay is to test hits from the Primary Screening Assay (Steps 47-54) to eliminate false positives due to compound fluorescence that increases between the 0 min and 90 min reads in the assay. One cause of the increase in compound fluorescence …
WebSep 1, 2013 · The counterscreen protocol selected for HTS is identical to the primary HTS assay protocol with the exception that the hV2R[L 83 Q] cells are incubated in the …
WebTo look for specificity of the NanoBiT® Myc/Max assay, we used an unrelated p53-Mdm2 NanoBiT® assay as a counterscreen. Nutlin-3, a specific p53-Mdm2 interaction inhibitor, disrupted the PPI between p53 and Mdm2 in this assay, as shown in Figure 7. We also tested nutlin-3 in cells expressing Myc- and Max- NanoBiT® constructs, which did not ... ahs isolation guidelineshttp://www.genomicglossaries.com/CONTENT/Assays.asp ahs lippincottWebTable 2 Overview of PubChem assays for GPR7 antagonist project. AID Assay Name Tested/Active Non-ML Tested Purch Tested/Active ... ahs lippincott advisorWebAssay Overview: The purpose of this assay was to determine S1P1 agonist dose response for compounds identified as active in the uHTS S1P1 receptor counterscreen assay . The assay was performed as … ahs ivig dose calculatorWebFeb 24, 2010 · The S1P5 receptor counterscreen assay is in the same TANGO format as the S1P4 receptor assay used for the primary screening based upon arrestin recruitment to activated receptor. From this we infer … ahs male pregnancyWebBioAssay record AID 624051 submitted by The Scripps Research Institute Molecular Screening Center: Counterscreen assay for antagonists of the orexin 1 receptor … ahs medical suppliesahs mandate vaccines