Elf5-cre

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August undefined, 2024

WebHomozygous null mice display embryonic lethality. Embryos homozygous for one null allele failed to develop extraembryonic ectoderm, did not maintain trophoblast stem cells, and had abnormal or absent mesoderm. Mice heterozygous for another allele had impaired lactation and mammary gland development. Gene Ontology. (GO) WebThe transcription factor E74-like factor 5 (Elf5) functions downstream of the prolactin receptor signaling pathway and plays an important role in mammary gland development. Using conditional mouse knockouts, we have previously shown that Elf5-null mammary glands exhibit a complete failure of alveolo …

Elf5 counteracts precocious trophoblast differentiation by …

WebApr 19, 2024 · Generation of Elf5 Cre ERT 2-GFP mice. (A) Schematic diagram of the strategy followed to insert Cre-ERT2-GFP gene segment in second exon of Elf5 gene. (B) PCR image showing presence of Cre … WebApr 19, 2024 · Moreover, haploinsufficiency of Elf5 limits the use of current mouse models to investigate adult tissue distribution of Elf5. Here, we successfully generated Elf5 Cre ERT 2- GFP bacterial artificial chromosome (BAC) transgenic mice and show that Elf5 + cells are present in several adult tissues, where its expression was previously not known. most popular music radio stations

Expression of Cre recombinase in early diploid ... - ResearchGate

WebMay 15, 2009 · However, the Elf5 in K14-Cre/Elf5 +/f mammary tissue was expressed in a mosaic pattern (Fig. 6 E) and complete ablation of Elf5 expression was confirmed in K14-Cre/Elf5 f/f mammary epithelium (Fig. 6 I). To determine whether alterations in PrlR/Jak2/Stat5 signaling pathway are responsible for the failed lobuloalveolar … http://www.rpb.ioz.cas.cn/yjz/wangyanling/ WebOct 3, 2024 · The ablation of Sirt1 protein in Elf5 cre/+, Sirt1 fl/fl mice was first confirmed by IF staining (Figure 5b), and western blotting indicated that compared with those in the placentas of Sirt1 fl/fl littermates, in addition to increased acetylation of P53, the levels of both the P53 and P21 proteins were increased in Elf5 cre/+, Sirt1 fl/fl ... mini golf in brighton

Elf5 Regulates Mammary Gland Stem/Progenitor Cell Fate by …

ELF5 inhibits the proliferation and invasion of breast …

WebGenerally taller than humans, elves possess a graceful, slender physique that is accentuated by their long, pointed ears. It is a mistake, however, to consider them weak … WebTherefore, in mice (Elf5-Cre knock-in), the expression of Cre recombinase driven by the Elf5 promoter can be used to study genes specifically and efficiently in the trophoblast lineage during ... mini golf in brickellWebAug 15, 2024 · The transcription factor ELF5 (ELF5) shows robust and directionally consistent associations across different outcome definitions, including a >4-fold higher risk (odds ratio: 4.88; 95%-CI: 2.47-9.63; p-value < 5.0 × 10 -6) for severe COVID-19 per 1 s.d. higher genetically predicted plasma ELF5. We show that ELF5 is specifically expressed … mini golf in bull creek

"WebMar 19, 2024 · When ELF5-6KR plus Myc-Ub were co-expressed in HEK293T cells, the mutant ELF5 protein exhibited a lower basal level of ubiquitination than its wild-type counterpart and the ELF5-6KQ mutant, an ... " - Elf5-cre

Elf5-cre

Elf5 inhibits the epithelial–mesenchymal transition in

http://genesdev.cshlp.org/content/23/20/2382.full WebSep 10, 2010 · To further probe the effects of loss of Elf5 during pregnancy, we compared mammary glands from K14-Cre/Elf5 f/f animals and their wildtype type littermate controls …

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WebReal-time RT–PCR analyses demonstrated that the levels of Elf5 mRNA were lower in Stat5a/5b fl/fl;Cre compared with Stat5a/5b fl/fl, and that Elf5 mRNA was induced to a greater extent by prolactin in mammary tissue of Stat5a/5b fl/fl mice as compared with Stat5a/5b fl/fl;Cre mice (Supplemental Fig. 4), indicating cell- and stage-specific ...

WebElf5 knockout mice are embryonically lethal, limiting in vivo studies pertaining to its function. Moreover, haploinsufficiency of Elf5 limits the use of current mouse models to … WebThe Elf5-null mice have been described previously [14]. Mice of C57BL/6 strain (6–8 generations) were used for experi-ments with virgin mammary glands. The genotype of the con-trol wild-type (WT) animals was either K14-Cre/Elf5þ/þ or Elf5f/f, whereas the genotype of the Elf5 conditional knock-outs (Elf5-null) was K14-Cre/Elf5f/f. Animal ...

WebS. Singh et al. Generation of a new Elf5-Cre BAC mouse model. Results and Discussions Construct design and generation of Elf5CreERT2-GFP mice To develop Elf5CreERT2 … WebApr 4, 2024 · Tg (Ggt1-cre)M3Egn - Renal & Urinary SystemRecombinase Activity Detail. The rat proximal promoter to Ggt1 was used in a transgene construct to drive the expression of cre recombinase, followed by a termination sequence from human growth hormone. Cre expression was limited to cortical tubular epithelium of the kidney, and was first detected ...

WebOct 21, 2012 · The genotype of the control WT animals was either K14-Cre/Elf5 +/+ or Elf5 f/f whereas Elf5 conditional knockouts (referred to as Elf5-KO) were K14-Cre/Elf5 f/f. Animal procedures were conducted ...

WebSynonym: Elf5-Cre Molecular description: CRISPR/Cas-mediated genome engineering T2A in front of the Cre coding sequence was inserted into the last coding exon of Elf5, immediately before the stop codon. mini golf in canterburyWebApr 20, 2012 · The Elf5-null mice have been described previously . Mice of C57BL/6 strain (6–8 generations) were used for experiments with virgin mammary glands. The genotype … most popular music websitesWebSep 20, 2024 · The Elf 5e (5th Edition) in DnD Races. The magical people of the otherworldly grace are called as Elf 5e Race. They are not the entire part and should be … mini golf in carlisleWebOct 5, 2008 · Epigenetic silencing of Elf5 by DNA methylation interrupts this trophoblast-specific reinforcement loop in the embryonic lineage, and thereby safeguards embryonic cells from differentiating into ... mini golf in cape may njWebAug 15, 2014 · The Elf5-/FLEx and Elf5+/FLEx ES cells were transiently transfected with a Cre construct which resulted in the rearrangement of the FLEx allele leading to the … most popular mystery authors 2020WebApr 1, 2024 · Neonatal mouse kidneys deficient for Elf5 expression (Cdh16->Cre; Elf5*/f, where *= floxed or null allele) express 25% less Aqp2 and 25% less Avpr2 compared … mini golf in brisbaneWebOct 5, 2008 · Epigenetic silencing of Elf5 by DNA methylation interrupts this trophoblast-specific reinforcement loop in the embryonic lineage, and thereby safeguards embryonic … mini golf in city walk