WebPhenyl Sepharose 6 Fast Flow is a well-established, standard aromatic HIC medium for capture and intermediate purification requiring low to medium hydrophobicity. Provide convenient and fast small-scale protein purifications Two levels of substitution on Phenyl Sepahrose 6 Fast Flow help find optimal selectivity and binding capacity WebProduct Introduction. Phenyl-6FF (HS) can interact with some hydrophobic protein or antibody under high ionic strength conditions (high ionic strength may increase the interaction between ligand and hydrophobic groups), thus to achieve the purpose of separation and purification. Phenyl-6FF (HS) is mainly used for capture of initial samples …
Capto™ Phenyl (high sub), Capto™ Butyl, Capto™ Octyl
WebApr 15, 2024 · The Phenyl Sepharose HP elution product was then dialyzed into 50 mM glycine, pH 9.5 using a 3000 NMWC hollow fiber filter in preparation for loading onto a Q Sepharose FF column. WebOct 12, 2016 · Phenyl-Sepharose is often classified as a weak “hydrophobic gel” and should therefore be used to bind strongly hydrophobic proteins such as membrane-bound enzymes and receptors. This is because of the nature of hydrophobic binding, which is a much stronger type of interaction than ionic binding. grants for literacy nonprofits
Phenyl Sepharose CL-4B
WebPhenyl Sepharose 6 Fast Flow (high sub) Max. Pressure: 5 bar [0.5 MPa] (70 psi) Height (English) 0.984 in. 4 Cytiva HiTrap™ Phenyl HP Columns. Prepacked columns for convenient and fast separations. Cytiva Lifescience™ HiTrap™ Phenyl HP Columns are designed for use with a syringe, laboratory pump or chromatography system. ... WebQuantitative determination of the ligand content in Benzamidine Sepharose 4 Fast Flow media with ion-pair chromatography. J. Gustavsson, J. Färenmark, B. Johansson; Chemistry. ... Quantitative determination of the ligand in phenyl-sepharose FF with proton nuclear magnetic resonance and derivative ultraviolet spectroscopy. B. Johansson, I. Drevin; grants for llc 2023