Phospho ampk antibody cst
WebThe Phospho-AMPKalpha (Thr172) Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody to PRKAA1, and PRKAA2. This antibody recognizes Human, Mouse, Rat, and Non-Human Primate antigen. The Phospho-AMPKalpha (Thr172) Antibody has been validated for the following applications: Western Blot. Webetary methodology from Cell Signaling Technology for peptide enrichment by immunoprecipitation using a specific bead-conjugated antibody in conjunction with liquid
Phospho ampk antibody cst
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Web® 2015 Cell Signaling Technology, Inc. Orders ff 77-1-CELL (255) orderscellsignal.com Support 77-7-TECH (2) infocellsignal.com Web ff www.cellsignal.com page 2 of 2 9957 For western blots, incubate membrane with diluted primary antibody in either 5% w/v BSA or nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight. WebThe Human Phospho-Kinase Array (Catalog # ARY003B) is a rapid, sensitive, and economical tool to simultaneously detect the relative site-specific phosphorylation of 43 kinases and 2 related total proteins without performing …
WebAMPK is a heterotrimer consisting of a catalytic α-subunit and two regulatory subunits, β and γ. Each subunit exists as multiple isoforms (α 1, α 2, β 1, β 2, γ 1, γ 2, and γ 3 ), giving 12 different possible combinations of holoenzyme with different tissue distribution and subcellular localization ( 2 , 3 ). WebDescription: The AMPK and ACC Antibody Sampler Kit provides an economical means to investigate energy homeostasis and fatty acid synthesis within the cell. The kit includes …
WebAnti-phospho-AMPK α (Thr172) Antibody is an antibody against phospho-AMPK α (Thr172) for use in WB. Anti-phospho-AMPK α (Thr172) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and … WebThe primary antibody to LC3B (ab192890, Abcam, Cambridge, MA, UK) or Cleaved Caspase 3 (#9664, CST, MA, USA) was added dropwise to the sections and incubated overnight at 4 °C. Next day, slices were washed three times in PBS before incubation with secondary antibody (A0208, Bain-marie Biotech, Shanghai, China) for 1 h at room temperature.
WebThis antibody has been shown to work in applications such as: Precipitation, Immunoprecipitation, and Western Blot. The AMPKalpha Antibody was generated using …
WebApr 5, 2024 · The rabbit anti-AMPK antibody (D5A2), rabbit anti-SIRT1 antibody (D1D7), rabbit anti-Phospho-FoxO1 (Ser256), and rabbit anti-Phospho-AMPK (Thr172) were purchased from Cell Signaling Technology (Danvers, MA, USA). The RIPA lysate was purchased from Kangwei Century Biotechnology (Cwbio, Beijing, China). highland cargo matWebo Anti-phospho-AMPK-alpha (Thr172) antibody; Cell Signaling #2531 OR Anti-phospho-AMPK-alpha (Thr172) (40H9) Rabbit mAB; Cell Signalling #2535. o Anti-AMPK-alpha … how is bismuth rainbowWebMar 25, 2024 · As an energy sensor, adenosine monophosphate-activated protein kinase (AMPK) plays an important role in activating autophagy by inhibiting mammalian target of rapamycin (mTOR) [ 15 ]. Although AMPK is a key modulator of autophagy, it is less clear whether AMPK is involved in SQSTM1-dependent autophagy to initiate clearance of Keap1 … highland cargo sweatpantsWebJun 23, 2024 · Specifically, metformin can activate AMP-activated protein kinase (AMPK) to inactivate mammalian target of rapamycin (mTOR), change intracellular reactive oxygen species (ROS) levels, and inhibit mitochondrial functions [ 4 ]. Metformin has also been shown to regulate iron homeostasis in cells, which may be linked to cell metabolism [ 5 ]. highland cargo barWebPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr172 of human AMPKα. … how is bisoprolol excretedWebPhospho-AMPK alpha-1,2 (Thr183, Thr172) Polyclonal Antibody Advanced Verification This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen … highland car salesWebSnf1 localization was observed in formaldehyde-fixed cells immunostained with anti-phospho-T172-AMPK antibody (1:50, Cell Signalling) and with TRITC-conjugated anti-rabbit antibody (1:100, Life Technologies). For the colocalization experiment, Cdc3-HA was detected with 16B12 monoclonal antibody (1:200, Babco) and with FITC-conjugated anti … highland capital bishkek